What does amylase hydrolyze

Citations 4. Cited By. This article is cited by 4 publications. Sehrish Maqsood, Samuel M. Kilpatrick, Chloe D.

Truong, Scott R. Journal of Chemical Education , 98 3 , Incorporating Carbohydrates into Laboratory Curricula. Chemical Reviews , 17 , Journal of Chemical Education , 95 8 , Pair your accounts. Your Mendeley pairing has expired. Please reconnect. This website uses cookies to improve your user experience. In order to use these starches as a carbon source, bacteria must secrete a-amylase and oligo-1,6-glucosidase into the extracellular space.

These enzymes break the starch molecules into smaller glucose subunits which can then enter directly into the glycolytic pathway.

In order to interpret the results of the starch hydrolysis test, iodine must be added to the agar. The iodine reacts with the starch to form a dark brown color. Thus, hydrolysis of the starch will create a clear zone around the bacterial growth.

Bacillus subtilis is positive for starch hydrolysis pictured below on the left. The organism shown on the right is negative for starch hydrolysis. University Ave.